S-YTSβ

Saccharomyces - Yeast Thyroid β Screen

Detection of thyroid receptor β mediated effects

120 €
Test Specification
Duration of Assay
ca. 22 h
Number of Samples
max. 40
Number of Samples (LID)
not applicable
Validation
currently in progress
Calibration Range
0 - 50 µg/L (T3)
Limit of Detection
ca. 1.0 µg/L (T3)
Concerned about data analysis?

 

 

 

 

 

 

  •  Comes with our test kits
  •  Web tool with free access
  •  Simple and intuitive use
  •  Statistical assessment of results
  •  Comprehensive report with graphics
  •  Customized report available

 

Further documents 

 

S-YTSbeta data sheet

 

Technical requirements

 

 

Available test kit format

 

 

TestKitFormat
Other kits

Product Description
The S-YTSβ is an effect-based 96well assay for the the determination of thyroid receptor β mediated effects expressed as T3EQs (triiodothyronine equivalent concentration) in aqueous samples by means of an optimized yeast biosensor based on Saccharomyces cerevisiae. The original procedure was developed and published by Shiizaki et. al*. The new_diagnostics S-YTSβ uses a different Saccharomyces cerevisiae strain with improved properties. No sterile conditions are required for the whole test procedure.
*Shiizaki K, Asai S, Ebata S, Kawanishi M, Yagi T (2010) Establishment of yeast reporter assay systems to detect ligands of thyroid hormone receptors alpha and beta. Toxicol In Vitro 24:638-644


Key Characteristics

The S-YTSβ  determines the T3EQ (triiodothyronine equivalents) of aqueous samples.

Validation and Standardization

A factorial in-house validation is in progress for method validation.


 

Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.

Support

Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

 

Type of samples
Aqueous extracts
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Wastewater
For substance testing

Test Principle

Genetic Modifications

This yeast strain contains the gene for the human thyroid receptor β, the gene for the steroid receptor coactivator 1e (src1e), specific gene regulation sequences and the reporter gene lacZ.

Transactivation

Thyroid active substances bind to the thyroid receptors and the resulting homodimers trigger the transcription of the reporter gene.


 

Calibration

The reference standard for calibration and quantification is triiodothyronine (T3). The dose-response curve corresponds to a non-linear model.

Detection

The detection is performed after reaction of the reporter enzyme β-galactosidase with the chromogenic substrate CPRG at 580 nm.

general scheme of the biosensor
#29303B