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S-YESᴹᴰ

Saccharomyces - Yeast Estrogen Screen (strain McDonnell)

Detection of estrogenic effects

 

from 200 €
Test Specification
Duration of Assay
ca. 20 h
Number of Samples
max. 80 (S-YESᴹᴰ plus) (EEQ)
Number of Samples (LID)
non-applicable
Validation
in-house
Calibration Range
0 - 400 ng/L (E2)
Limit of Detection
3.8 ng/L (E2)
Concerned about data analysis?

 

 

 

 

 

 

  •  Comes with our test kits
  •  Web tool with free access
  •  Simple and intuitive use
  •  Statistical assessment of results
  •  Comprehensive report with graphics
  •  Customized report available

 

Further documents

 

S-YESᴹᴰ data sheet

 

Technical Requirements

 

 

Available test kit format

 

 

Other kits

Product Description
The S-YESᴹᴰ (Saccharomyces-Yeast Estrogen Screen) is an effect-based 96well assay for the detection of estrogenic activity in various types of water samples. The S-YESᴹᴰ uses the conventional and recombinant yeast Saccharomyces cerevisiae as the estrogen responsive biosensor*. No sterile conditions are required for the whole test procedure. 
*McDonnell DP et al.; High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae; Steroid Biochem. Mol. Biol.; Sep. 1991; 39(3); 291-297

 

 

Key Characteristics

The S-YESᴹᴰ determines the EEQ (17β-Estradiol Equivalents) of water samples especially such with limited volume and organic solvent up to 15 %.

Validation and Standardization

A factorial in-house validation was performed for method validation of the S-YESᴹᴰ.

 
Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL® for the statistical analysis and reporting of your test results.

Support
Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

 

Applications
Extracts with solvent concentration up to 15 %
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Waste water

Test Principle
Genetic Modifications

The yeast carries a plasmid with a cassette including the human estrogen receptor gene (hERα) and a plasmid with a reporter gene cassette including the lacZ gene.

Transactivation

Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.

 
Calibration

The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate Chlorophenol Red-β-D-galactopyranoside at 580 nm.

Detection

The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.

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