Saccharomyces - Yeast Estrogen Screen (strain McDonnell)
Detection of estrogenic effects
*McDonnell DP et al.; High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae; Steroid Biochem. Mol. Biol.; Sep. 1991; 39(3); 291-297
The S-YESᴹᴰ determines the EEQ (17β-Estradiol Equivalents) of water samples especially such with limited volume and organic solvent up to 15 %.
A factorial in-house validation was performed for method validation of the S-YESᴹᴰ.
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The yeast carries a plasmid with a cassette including the human estrogen receptor gene (hERα) and a plasmid with a reporter gene cassette including the lacZ gene.
Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate Chlorophenol Red-β-D-galactopyranoside at 580 nm.
The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.