Saccharomyces - Yeast Estrogen Screen (strain McDonnell)
Detection of estrogenic effects
The S-YESbasic (Saccharomyces-Yeast Estrogen Screen) is a simple and fast effect-based cuvette test for the detection of estrogenic activity in various types of water samples. The S-YESbasic uses the conventional and recombinant yeast Saccharomyces cerevisiae as the estrogen responsive biosensor. No sterile conditions are required for the whole test procedure.
The S-YESbasic determines the EEQ (17β-Estradiol Equivalents) of water samples. The test requires only basic lab equipment and the test duration is only approx. 4 hours.
A factorial in-house validation was performed for method validation of the S-YESbasic.
Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.
The yeast carries a plasmid with a cassette including the human estrogen receptor gene (hERα) and a plasmid with a reporter gene cassette including the lacZ gene.
Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.
The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate Chlorophenol Red-β-D-galactopyranoside at 580 nm.