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S-YESbasic

Saccharomyces - Yeast Estrogen Screen (strain McDonnell)

Detection of estrogenic effects 

 

from 130 €
Test Specification
Duration of Assay
ca. 4 h
Number of Samples
6 with 2 replicates (EEQ)
Number of Samples (LID)
-
Validation
in-house
Calibration Range
0 - 400 ng/L (E2)
Limit of Detection
12.8 ng/L (E2)
Concerned about data analysis?

 

 

 

 

 

 

  •  Comes with our test kits
  •  Web tool with free access
  •  Simple and intuitive use
  •  Statistical assessment of results
  •  Comprehensive report with graphics
  •  Customized report available

 

Further documents

 

S-YESbasic data sheet

 

Technical Requirements

 

 

 

Other kits

Product Description

The S-YESbasic (Saccharomyces-Yeast Estrogen Screen) is a simple and fast effect-based cuvette test for the detection of estrogenic activity in various types of water samples. The S-YESbasic uses the conventional and recombinant yeast Saccharomyces cerevisiae as the estrogen responsive biosensor. No sterile conditions are required for the whole test procedure.

 

Key Characteristics

The S-YESbasic determines the EEQ (17β-Estradiol Equivalents) of water samples. The test requires only basic lab equipment and the test duration is only approx. 4 hours.

Validation and Standardization

A factorial in-house validation was performed for method validation of the S-YESbasic.

 
Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL® for the statistical analysis and reporting of your test results.

Support
Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

 

Applications
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Waste water

Test Principle
Genetic Modifications

The yeast carries a plasmid with a cassette including the human estrogen receptor gene (hERα) and a plasmid with a reporter gene cassette including the lacZ gene.

Transactivation

Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.

 
Calibration

The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.

Detection

The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate Chlorophenol Red-β-D-galactopyranoside at 580 nm.

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