from 370 €
A-YBS
Arxula - Yeast Bisphenol Screen
Detection of estrogenic effects
Concerned about data analysis?
- Comes with our test kits
- Web tool with free access
- Simple and intuitive use
- Statistical assessment of results
- Comprehensive report with graphics
- Customized report available
Further documents
Available test kit format
Product Description
The A-YBS (Arxula-Yeast Bisphenol Screen) is an effect-based 96well assay for the detection of cumulative activity of Bisphenols in various types of water samples. The A-YBS uses the non-conventional and recombinant yeast Arxula adeninivorans as the bisphenol responsive biosensor. The original biosensor based on Saccharomyyces cerevisiae was developed and published by Rajasärkkä et. al*. No sterile conditions are required for the whole test procedure.
*Johanna Rajasärkkä, Marko Virta; Characterization of a Bisphenol A Specific Yeast Bioreporter Utilizing the Bisphenol A-Targeted Receptor; Anal. Chem., 2013, 85 (21), pp 10067–10074
Key Characteristics
The A-YBS determines the BPAEQ (bisphenol A Equivalents) of aqueous samples. This test is sensitive and specific for the detection of bisphenols especially bisphenol A and bisphenol Z.
Validation and Standardization
A factorial in-house validation was performed for method validation of the A-YBS.
Data Evaluation
Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.
Support
Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.
Type of samples
Aqueous extracts
Ultrapure-, drinking- and mineral water
Surface water
Ground water and well water
Wastewater
Test Principle
Genetic Modifications
In the yeast genome the gene for directly modificated1 human estrogen receptor (hERα) and a reporter gene cassette have been integrated.
Transactivation
Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
Calibration
The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.
Detection
The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.
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