Arxula - Yeast Glucocorticoid Screen
Detection of glucocorticoid effects
The A-YGS determines the CEQ (Corticosterone Equivalents) of aqueous samples.
A factorial in-house validation was performed for method validation of the A-YGS.
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In the yeast genome the gene for the human glucocorticoid receptor and a reporter gene cassette have been integrated.
Glucocorticoids bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
The reference standard for calibration and quantification is Corticosterone (CN). The dose-response curve corresponds to a non-linear model.
The detection is performed after reaction of the reporter enzyme phytase with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.