Arxula - Yeast Estrogen Screen
Detection of estrogenic activity
The A-YESlumi determines the EEQ (17β-Estradiol Equivalents) in aqueous samples with minimal requirement of sample volume. The assay has a superior sensitivity and can be used as a fast-screening test prior to chemical analysis.
A factorial in-house validation was performed for method validation of the A-YESlumi.
Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.
Will this test fits to my purposes? We give support for choosing the sufficient test for your application as well as before and during test establishment and for data evaluation.
In the yeast genome the gene for the human estrogen receptor (hERα) and a reporter gene cassette have been integrated.
The detection of light is performed after reaction of the reporter enzyme luciferase with the substrate luciferin.
Estrogens and estrogen-like substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.