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A-YBSlumi

Arxula - Yeast Bisphenol Screen luminescent

Detection of estrogenic effects

 

250 €
Test Specification
Duration of Assay
ca. 4.5 h
Number of Samples
max. 40 (BPAEQ)
Number of Samples (LID)
non-applicable
Validation
in-house
Calibration Range
0 - 200 µg/L (BPA)
Limit of Detection
7.3 µg/L (BPA)
Concerned about data analysis?

 

 

 

 

 

 

  •  Comes with our test kits
  •  Web tool with free access
  •  Simple and intuitive use
  •  Statistical assessment of results
  •  Comprehensive report with graphics
  •  Customized report available

 

Further documents

 

A-YBSlumi data sheet

 

Technical requirements

 

 

Available test kit format

 

 

Other kits

Product Description
The A-YBSlumi (Arxula-Yeast Bisphenol Screen) with luminescence detection is an effect-based 96well assay for the fast and sensitive determination of the cumulative activity of Bisphenols in various types of aqueous samples. The A-YBSlumi uses the non-conventional and recombinant yeast Arxula adeninivorans as a bisphenol responsive biosensor. No sterile conditions are required for the whole test procedure.
 
Key Characteristics

The A-YBSlumi determines the BPAEQ (bisphenol A equivalents) of aqueous samples with a less use of sample volume. This test is sensitive and specific for the detection of bisphenols especially bisphenol A and bisphenol Z.

Validation and Standardization

A factorial in-house validation was performed for method validation of the A-YBSlumi.

 
Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.

Support

Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

Type of samples
Aqueous extracts
Ultrapure-, drinking- and mineral water
Surface water
Ground water and well water
Wastewater

Test Principle
Genetic Modifications

In the yeast genome the gene for directly modificated1 human estrogen receptor (hERα) and a reporter gene cassette have been integrated.

Transactivation

Bisphenols bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.

 
Calibration

The reference standard for calibration and quantification is bisphenol A (bpA). The dose-response curve corresponds to a non-linear model.

Detection

The detection of light is performed after reaction of the reporter enzyme luciferase with the substrate luciferin.

 
1 Johanna Rajasärkkä, Marko Virta; Characterization of a Bisphenol A Specific Yeast Bioreporter Utilizing the Bisphenol A-Targeted Receptor; Anal. Chem., 2013, 85 (21), pp 10067–10074
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