Arxula - Yeast Bisphenol Screen luminescent
Detection of estrogenic effects
The A-YBSlumi determines the BPAEQ (bisphenol A equivalents) of aqueous samples with a less use of sample volume. This test is sensitive and specific for the detection of bisphenols especially bisphenol A and bisphenol Z.
A factorial in-house validation was performed for method validation of the A-YBSlumi.
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In the yeast genome the gene for directly modificated1 human estrogen receptor (hERα) and a reporter gene cassette have been integrated.
Bisphenols bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
The reference standard for calibration and quantification is bisphenol A (bpA). The dose-response curve corresponds to a non-linear model.
The detection of light is performed after reaction of the reporter enzyme luciferase with the substrate luciferin.