Arxula - Yeast Bisphenol Screen
Detection of estrogenic effects
The A-YBSlumi determines the BPAEQ (Bisphenol A Equivalents) of aqueous samples with a less use of sample volume. This test is sensitive and specific for the detection of bisphenols especially bisphenol A and bisphenol Z.
A factorial in-house validation was performed for method validation of the A-YBSlumi.
Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.
Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.
In the yeast genome the gene for modificated1 human estrogen receptor (hERα) and a reporter gene cassette have been integrated.
Bisphenols bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
The reference standard for calibration and quantification is bisphenol A (bpA). The dose-response curve corresponds to a non-linear model.
The detection of light is performed after reaction of the reporter enzyme luciferase with the substrate luciferin.