Arxula - Yeast Progesterone Screen
Detection of progestogenic effects
The A-YPS determines the PEQ (Progesterone Equivalents) of water samples with high matrix load.
A factorial in-house validation was performed for method validation of the A-YPS.
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In the yeast genome the gene for human progesterone receptor β (hPRβ) and a reporter gene cassette have been integrated.
Progestogens and progestogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
The reference standard for calibration and quantification is Progesterone. The dose-response curve corresponds to a non-linear model.
The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.