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A-YES®

Arxula - Yeast Estrogen Screen

Detection of estrogenic effects

 

from 350 €
Test Specification
Duration of Assay
ca. 26 h
Number of Samples
max. 40 (EEQ)
Number of Samples (LID)
max. 4
Validation
interlaboratory trial
Calibration Range
0 – 80 ng/L (E2)
Limit of Detection
1.8 ng/L (E2)
Concerned about data analysis?

 

 

 

 

 

 

  •  Comes with our test kits
  •  Web tool with free access
  •  Simple and intuitive use
  •  Statistical assessment of results
  •  Comprehensive report with graphics
  •  Customized report available

 

Further documents

 

A-YES data sheet

 

Technical Requirements

 

 

Available test kit format

 

 

Other kits

Product Description
The A-YES (Arxula-Yeast Estrogen Screen) is an effect-based 96well assay for the detection of estrogenic activity in various types of water samples. The A-YES uses the non-conventional and recombinant yeast Arxula adeninivorans as an estrogen responsive biosensor.
 
Key Characteristics

The A-YES is a robust assay for analyzing EEQ (17β-Estradiol Equivalents) and LID (Lowest Ineffective Dilution) of samples especially with high matrix load or with high salt content. No sterile conditions are required for the whole test procedure.

Validation and Standardization

The A-YES is a standardized method according to ISO 19040-2:2018. An international interlaboratory trial was performed for method validation.

 
Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL® for the statistical analysis and reporting of your test results.

Support

Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

Applications
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Sewage
Seawater and brackish water
Aqueous extracts
For substance testing

Test Principle
Genetic Modifications

In the yeast genome the gene for the human estrogen receptor (hERα) and a reporter gene cassette have been integrated.

Transactivation

Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.

 
Calibration

The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.

Detection

The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate.

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