Arxula - Yeast Dioxin Screen
Detection of Ah-Receptor mediated effects
The A-YDS determines the NEQ (β-Naphthoflavone Equivalents) of water samples.
An in-house validation was performed for method validation.
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In the yeast genome the genes for the human aryl-hydrocarbon receptor (hAhR) and the human aryl-hydrocarbon receptor nuclear translocator (hARNT) and a reporter gene cassette have been integrated.
In the presence of hAhR ligands, heterodimeric complexes are formed which act as transcription factors for the transcription of the reporter gene.
Calibration The reference standard for calibration and quantification is β-Naphthoflavon. The dose-response curve corresponds to a non-linear model.
The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.