Skip to main content

A-YDS

Arxula - Yeast Dioxin Screen

Detection of Ah-Receptor mediated effects

 

from 250 €
Test Specification
Duration of Assay
ca. 26h
Number of Samples
max. 40 (NEQ)
Number of Samples (LID)
non-applicable
Validation
in-house
Calibration Range
0-10.000 ng/L (β-Naphthoflavon)
Limit of Detection
59.0 ng/L (β-Naphthoflavon)
Concerned about data analysis?

 

 

 

 

 

 

  •  comes with our test kits
  •  web tool with free access
  •  simple and intuitive use
  •  statistical assessment of results
  •  comprehensive report with graphics
  •  customized report available

 

Further documents

 

A-YDS data sheet

 

Technical Requirements

 

 

Available test kit format

 

 

Other kits

Product Description
The A-YDS (Arxula-Yeast Dioxin Screen) is an effect-based 96well assay for the detection of aryl hydrocarbon receptor mediated activity in surface water and aqueous extracts. The A-YDS uses the non-conventional and recombinant yeast Arxula adeninivorans as the responsive biosensor for aryl hydrocarbon receptor mediated activity. No sterile conditions are required for the whole test procedure.
 
Key Characteristics

The A-YDS determines the NEQ (β-Naphthoflavone Equivalents) of water samples. The test is especially for.

Validation and Standardization

An in-house validation was performed for method validation.

 
Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL® for the statistical analysis and reporting of your test results.

Support

Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

Applications
Determination aryl hydrocarbon receptor mediated effects of drug candidates
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Sewage
Aqueous extracts

Test Principle
Genetic Modifications

In the yeast genome the genes for the human aryl-hydrocarbon receptor (hAhR) and the human aryl-hydrocarbon receptor nuclear translocator (hARNT) and a reporter gene cassette have been integrated.

Transactivation

In the presence of hAhR ligands, heterodimeric complexes are formed which act as transcription factors for the transcription of the reporter gene.

 
Calibration

Calibration The reference standard for calibration and quantification is β-Naphthoflavon. The dose-response curve corresponds to a non-linear model.

Detection

The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.

url(/sites/default/files/pictures/Header/Header-Produkte_0.1.jpg)