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A-YAS®

Arxula-Yeast Androgen Screen

Detection of androgenic effects

 

from 300 €
Test Specification
Duration of Assay
ca. 26 h
Number of Samples
max. 40 (DHTEQ)
Number of Samples (LID)
non-applicable
Validation
in-house
Calibration Range
0 - 360 ng/L (DHT)
Limit of Detection
31.3 ng/L (DHT)
Concerned about data analysis?

 

 

 

 

 

 

  •  Comes with our test kits
  •  Web tool with free access
  •  Simple and intuitive use
  •  Statistical assessment of results
  •  Comprehensive report with graphics
  •  Customized report available

 

Further documents

 

A-YAS data sheet

 

Technical Requirements

 

 

Available test kit format

 

 

Other kits

Product Description
The A-YAS® (Arxula-Yeast Androgen Screen) is an effect-based 96well assay for the detection of androgenic activity in various types of water samples. The A-YAS® uses the non-conventional and recombinant yeast Arxula adeninivorans as the androgen responsive biosensor. No sterile conditions are required for the whole test procedure.
 
Key Characteristics

The A-YAS® determines the DHTEQ (5α-Dihydrotestosterone Equivalents) of samples with high matrix load.

Validation and Standardization

A factorial in-house validation was performed for method validation of the A-YAS®.

 
Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL® for the statistical analysis and reporting of your test results.

Support

Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

Applications
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Sewage
Aqueous extracts
and for substance testing

Test Principle
Genetic Modifications

In the yeast genome the gene for the human androgen receptor (hAR) and a reporter gene cassette have been integrated.

Transactivation

Androgens and androgenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.

 
Calibration

The reference standard for calibration and quantification is 5α-Dihydrotestosterone (DHT).The dose-response curve corresponds to a non-linear model.

Detection

The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.

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