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µA-YES

microArxula - Yeast Estrogen Screen

Detection of estrogenic effects

 

from 350 €
Test Specification
Duration of Assay
ca. 26 h
Number of Samples
max. 80 (EEQ)
Number of Samples (LID)
max. 8
Validation
Equivalence Test
Calibration Range
0 - 80 ng/L (E2)
Limit of Detection
2.3 ng/L (E2)
Concerned about data analysis?

 

 

 

 

 

 

  •  comes with our test kits
  •  web tool with free access
  •  simple and intuitive use
  •  statistical assessment of results
  •  comprehensive report with graphics
  •  customized report available

 

Further documents

 

microA-YES data sheet

 

Technical Requirements

 

 

Available test kit format

 

 

Other kits

Product Description
The µA-YES (microArxula-Yeast Estrogen Screen) is an effect-based 384well assay for the detection of estrogenic activity in various types of water. The µA-YES uses the non-conventional and recombinant yeast Arxula adeninivorans as the estrogen responsive biosensor. No sterile conditions are required for the whole test procedure.
 
Key Characteristics

The µA-YES determines the EEQ (17β-Estradiol Equivalents) and LID (Lowest Ineffective Dilution) of water samples especially such with limited volume like extracts. Only 40 µL of sample per well are required.

Validation and Standardization

The equivalence of µA-YES to the A-YES® is proven. The A-YES® is a standardized method according to ISO 19040-2:2018.

 
Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL® for the statistical analysis and reporting of your test results.

Support

Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

Applications
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Seawater and brackish water
Aqueous extracts

Test Principle
Genetic Modifications

In the yeast genome the gene for the human estrogen receptor (hERα) and a reporter gene cassette have been integrated.

Transactivation

Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.

 
Calibration

The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.

Detection

The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.

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