microArxula - Yeast Estrogen Screen
Detection of estrogenic effects
The µA-YES determines the EEQ (17β-Estradiol Equivalents) and LID (Lowest Ineffective Dilution) of water samples especially such with limited volume like extracts. Only 40 µL of sample per well are required.
The equivalence of µA-YES to the A-YES is proven. The A-YES is a standardized method according to ISO 19040-2:2018.
Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.
Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.
In the yeast genome the gene for the human estrogen receptor (hERα) and a reporter gene cassette have been integrated.
Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.
The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.