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microArxula - Yeast Estrogen Screen

Detection of estrogenic effects


from 350 €
Test Specification
Duration of Assay
ca. 26 h
Number of Samples
max. 80 (EEQ)
Number of Samples (LID)
max. 8
Equivalence Test
Calibration Range
0 - 80 ng/L (E2)
Limit of Detection
2.3 ng/L (E2)
Concerned about data analysis?







  •  comes with our test kits
  •  web tool with free access
  •  simple and intuitive use
  •  statistical assessment of results
  •  comprehensive report with graphics
  •  customized report available


Further documents


micro a-yes data sheet


Technical Requirements



Available test kit format



Other kits

Product Description
The µA-YES (microArxula-Yeast Estrogen Screen) is an effect-based 384well assay for the detection of estrogenic activity in various types of water. The µA-YES uses the non-conventional and recombinant yeast Arxula adeninivorans as the estrogen responsive biosensor. No sterile conditions are required for the whole test procedure.
Key Characteristics

The µA-YES determines the EEQ (17β-Estradiol Equivalents) and LID (Lowest Ineffective Dilution) of water samples especially such with limited volume like extracts. Only 40 µL of sample per well are required.

Validation and Standardization

The equivalence of µA-YES to the A-YES is proven. The A-YES is a standardized method according to ISO 19040-2:2018.

Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.


Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

Type of samples
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Seawater and brackish water
Aqueous extracts

Test Principle
Genetic Modifications

In the yeast genome the gene for the human estrogen receptor (hERα) and a reporter gene cassette have been integrated.


Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.


The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.


The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate p-nitrophenyl phosphate at 405 nm.