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A-YESᴴᵀ

Arxula - Yeast Estrogen Screen(High Throughput)

Detection of estrogenic effects

 

On Request
Test Specification
Duration of Assay
ca. 22 h
Number of Samples
max. 384 (EEQ)
Number of Samples (LID)
non-applicable
Validation
Test of applicability
Calibration Range
Depending on the respective application
Limit of Detection
non-applicable
Concerned about data analysis?

 

 

 

 

 

 

  •  comes with our test kits
  •  web tool with free access
  •  simple and intuitive use
  •  statistical assessment of results
  •  comprehensive report with graphics
  •  customized report available

 

Further documents

 

A-YESᴴᵀ data sheet

 

Technical Requirements

 

 

Available test kit format

 

 

Other kits

Product Description
The A-YESᴴᵀ (Arxula-Yeast Estrogen Screen for High Throughput) is an effect-based 384well assay for the detection of estrogenic activity in various types of water with low matrix load. The A-YESᴴᵀ uses the non-conventional and recombinant yeast Arxula adeninivorans as the estrogen responsive biosensor. No sterile conditions are required for the whole test procedure.
 
Key Characteristics

The A-YESᴴᵀ is a semi-quantitative assay with ready-to-use 384well test plates suitable for automatized test procedures.

Validation and Standardization

The applicability of A-YESᴴᵀ was proven by assessing various estrogens and substances with estrogenic potential.

 
Data Evaluation

Concerned about data analysis? Together with QuoData we will provide a solution for data analysis depending on your requirements.

Support

Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

Applications
Determination of potential estrogenic effects of drug candidates
Semi-quantitative analysis of ultrapure-, drinking- and mineral water
Semi-quantitative analysis of ground-, well- and surface water with low matrix effects

Test Principle
Genetic Modifications

In the yeast genome the gene for the human estrogen receptor (hERα) and a reporter gene cassette have been integrated.

Transactivation

Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.

 
Detection

After 18-22 h the fluorescence (excitation 535 nm, emission 595 nm) is measured directly in the test plate.

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