Arxula - Yeast Estrogen Screen(High Throughput)
Detection of estrogenic effects
The A-YESᴴᵀ is a semi-quantitative assay with ready-to-use 384well test plates suitable for automatized test procedures.
The applicability of A-YESᴴᵀ was proven by assessing various estrogens and substances with estrogenic potential.
Concerned about data analysis? Together with QuoData we will provide a solution for data analysis depending on your requirements.
Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.
In the yeast genome the gene for the human estrogen receptor (hERα) and a reporter gene cassette have been integrated.
Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
After 18-22 h the fluorescence (excitation 535 nm, emission 595 nm) is measured directly in the test plate.