Arxula - Yeast Androgen Screen (High Throughput)
Detection of androgenic effects
The A-YASᴴᵀ is a semi-quantitative assay with ready-to-use 384well test plates suitable for automatized test procedures.
The applicability of A-YASᴴᵀ was proven by assessing various estrogens and substances with estrogenic potential.
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In the yeast genome the gene for the human androgen receptor (hAR) and a reporter gene cassette have been integrated.
Androgens and androgenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
After 18-22 h the fluorescence (excitation 535 nm, emission 595 nm) is measured directly in the plate.