from 260 €
S-YESᴹᴰ
Saccharomyces - Yeast Estrogen Screen (strain McDonnell)
Detection of estrogenic effects
Concerned about data analysis?
- Comes with our test kits
- Web tool with free access
- Simple and intuitive use
- Statistical assessment of results
- Comprehensive report with graphics
- Customized report available
Further documents
Available test kit format
Product Description
The S-YESᴹᴰ (Saccharomyces-Yeast Estrogen Screen) is an effect-based 96well assay for the detection of estrogenic activity in various types of water samples. The S-YESᴹᴰ uses the conventional and recombinant yeast Saccharomyces cerevisiae as the estrogen responsive biosensor*. Two method protocols can be used for this test kit. A rapid screening of estrogenic effects of the samples can be achieved within approximately five to six hours. For better test sensitivity, the S-YESᴹᴰ can be performed overnight (approximately 18 h). No sterile conditions are required for the whole test procedure.
*McDonnell DP et al.; High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae; Steroid Biochem. Mol. Biol.; Sep. 1991; 39(3); 291-297
Key Characteristics
The S-YESᴹᴰ determines the EEQ (17β-Estradiol Equivalents) of water samples. Two method protocols can be applied for this test kit.
Validation and Standardization
A factorial in-house validation was performed for method validation of the S-YESᴹᴰ.
Data Evaluation
Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.
Support
Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.
Type of samples
Extracts with solvent concentration up to 15 %
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Waste water
Test Principle
Genetic Modifications
The yeast carries a plasmid with a cassette including the human estrogen receptor gene (hERα) and a plasmid with a reporter gene cassette including the lacZ gene.
Transactivation
Estrogens and estrogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
Calibration
The detection is performed photometrical after reaction of the reporter enzyme with the chromogenic substrate Chlorophenol Red-β-D-galactopyranoside at 580 nm.
Detection
The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.
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