A-YPSlumi

Arxula - Yeast Progesterone Screen

Detection of progestogenic effects

 

from 300 €
Test Specification
Duration of Assay
ca. 4.5 h
Number of Samples
max. 40 (PEQ)
Number of Samples (LID)
non-applicable
Validation
in-house
Calibration Range
0-500 ng/L (Progesterone)
Limit of Detection
8.5 ng/L (Progesterone)
Concerned about data analysis?

 

 

 

 

 

 

  •  comes with our test kits
  •  web tool with free access
  •  simple and intuitive use
  •  statistical assessment of results
  •  comprehensive report with graphics
  •  customized report available

 

Further documents

 

A-YPSlumi data sheet

 

Technical requirements

 

 

Available test kit format

 

 

TestKitFormat
Other kits

Product Description
The A-YPSlumi (Arxula-Yeast Progesterone Screen) with luminescence detection is an effect-based 96well assay for the detection of progestogenic activity in various types of water samples. The A-YPSlumi uses the non-conventional and recombinant yeast Arxula adeninivorans as the progesterone responsive biosensor. No sterile conditions are required for the whole test procedure.
 
Key Characteristics

The A-YPSlumi determines the PEQ (Progesterone Equivalents) of in aqueous samples with minimal requirement of sample volume. The assay can be used as a fast-screening test prior to chemical analysis.

Validation and Standardization

A factorial in-house validation was performed for method validation of the A-YPSlumi.

 
Data Evaluation

Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.

Support

Will this test fits to my purposes? We give sufficient support for choosing the right test for your application as well as before and during test establishment and for data evaluation.

Type of samples
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Sewage
Aqueous extracts
Substance testing

Test Principle
Genetic Modifications

In the yeast genome the gene for human progesterone receptor β (hPRβ) and a reporter gene cassette have been integrated.

Transactivation

Progestogens and progestogenically active substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.

 
Calibration

The reference standard for calibration and quantification is Progesterone. The dose-response curve corresponds to a non-linear model.

Detection

The detection of light is performed after reaction of the reporter enzyme luciferase with the substrate luciferin.

general scheme of the biosensor
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