300 €
A-YESlumi
Arxula - Yeast Estrogen Screen
Detection of estrogenic activity
Concerned about data analysis?
- Comes with our test kits
- Web tool with free access
- Simple and intuitive use
- Statistical assessment of results
- Comprehensive report with graphics
- Customized report available
Further documents
Available test kit format
Product Description
The A-YESlumi (Arxula-Yeast Estrogen Screen) with luminescence detection is an effect-based 96well assay for the fast and sensitive determination of the estrogenic activity in various types of aqueous samples. The A-YESlumi uses the non-conventional and recombinant yeast Arxula adeninivorans as an estrogen responsive biosensor. No sterile conditions are required for the whole test procedure.
Key Characteristics
The A-YESlumi determines the EEQ (17β-Estradiol Equivalents) in aqueous samples with minimal requirement of sample volume. The assay has a superior sensitivity and can be used as a fast-screening test prior to chemical analysis.
Validation and Standardization
A factorial in-house validation was performed for method validation of the A-YESlumi.
Data Evaluation
Concerned about data analysis? With the purchase of our test kits you get free access to BioVAL for the statistical analysis and reporting of your test results.
Support
Will this test fits to my purposes? We give support for choosing the sufficient test for your application as well as before and during test establishment and for data evaluation.
Type of samples
Aqueous extracts
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Wastewater
Test Principle
Genetic Modifications
In the yeast genome the gene for the human estrogen receptor (hERα) and a reporter gene cassette have been integrated.
Detection
The detection of light is performed after reaction of the reporter enzyme luciferase with the substrate luciferin.
Transactivation
Estrogens and estrogen-like substances bind to the receptors and the resulting complexes trigger the transcription of the reporter gene.
Calibration
The reference standard for calibration and quantification is 17β-Estradiol (E2). The dose-response curve corresponds to a non-linear model.
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