Publications

711, 2017

Separation and identification of hormone-active compounds using a combination of chromatographic separation and yeast-based reporter assay

November 7th, 2017|Comments Off on Separation and identification of hormone-active compounds using a combination of chromatographic separation and yeast-based reporter assay

Alexandre Chamas, Ha Thi Minh Pham, Martin Jähne, Karina Hettwer, Linda Gehrmann, JochenTuerk, Steffen Uhlig, Kirsten Simon, Kim Baronian, Gotthard Kunze Arxula adeninivorans-based yeast cell assays for the detection of steroid hormones demonstrated their efficiency for the determination of total hormone activity in a variety of samples using a microtiter plate format. In this study, a preliminary chromatographic separation using thin-layer chromatography plates is introduced in order to allow a rapid identification of the compounds responsible for this hormonal activity. The yeast whole cell assay can then be [...]

1910, 2017

Submitted: Validation of Arxula Yeast Estrogen Screen assay for detection of estrogenic activity in water samples: Results of an international interlaboratory study

October 19th, 2017|Comments Off on Submitted: Validation of Arxula Yeast Estrogen Screen assay for detection of estrogenic activity in water samples: Results of an international interlaboratory study

The publication of the results of the interlaboratory trial for the development of the new ISO standard 19040-2 for the A-YES® is currently under revision. http://new-diagnostics.com/wp-content/uploads/2017/10/Orangene-Zeitung-1.jpg

806, 2017

Simultaneous detection of three sex steroid hormone classes using a novel yeast-based biosensor

June 8th, 2017|Comments Off on Simultaneous detection of three sex steroid hormone classes using a novel yeast-based biosensor

Alexandre Chamas, Ha Thi Minh Pham, Martin Jähne, Karina Hettwer, Steffen Uhlig, Kirsten Simon, Almuth Einspanier, Kim Baronian, Gotthard Kunze A biosensor detecting estrogens, progestogens, and androgens in complex samples and in a single step is described. Three Arxula adeninivorans yeast strains were created, each strain producing a different recombinant human hormone receptor and a different fluorescent reporter protein. These strains were then mixed to create G1212/YRC102-hHR-fluo, the biological component of the biosensor. During incubation with G1212/YRC102-hHR-fluo, hormones present in a sample bind to their target receptor, which [...]

512, 2016

Recent published article in the LABO journal November 2016

December 5th, 2016|Comments Off on Recent published article in the LABO journal November 2016

Find our recent released and published specialist article about the topic " water- and wastewater analytics" in the LABO journal . In case of questions or interest feel free to contact us. Your new_diagnoistics team. http://new-diagnostics.com/wp-content/uploads/2016/02/home_auftragsanalysen.jpg

1408, 2015

Development of a recombinant Arxula adeninivorans cell bioassay for the detection of molecules with progesterone activity in wastewater.

August 14th, 2015|Comments Off on Development of a recombinant Arxula adeninivorans cell bioassay for the detection of molecules with progesterone activity in wastewater.

Alexandre Chamas & Annabel Nieter & Ha Thi Minh Pham & Martin Giersberg & Karina Hettwer & Steffen Uhlig & Kirsten Simon & Keith Baronian & Gotthard Kunze This study describes the development of a bioassay to detect the presence of progesterone and progesterone-like molecules in wastewater samples. The basis of the bioassay is the integration of the human progesterone receptor gene into the yeast Arxula adeninivorans for the constitutive synthesis of the receptor. After incubation, binding of the analyte to the receptor induces the production of a reporter [...]

402, 2015

The determination of pharmaceuticals in wastewater using a recombinant Arxula adeninivorans whole cell biosensor

February 4th, 2015|Comments Off on The determination of pharmaceuticals in wastewater using a recombinant Arxula adeninivorans whole cell biosensor

An Arxula adeninivorans based microbial biosensor has been developed for the determination of pharma- ceuticals and chemicals such as omeprazole, lansoprazole, ?-naphtoflavone and methylcholanthrene within 5 h using biochemical detection and 4 h and 10 min using amperometric detection. The biosen- sor consists of genetically modified A. adeninivorans G1212/YRC102-hAhR-hARNT-phyK (hAhR – human arylhydrocarbon receptor; hARNT – human arylhydrocarbon receptor nuclear translocator; inducible phyK – derived from Klebsiella sp. ASR1) as the biological component and coupled with either a bio- chemical or an amperometric detection method. The combination between hAh [...]

101, 2015

Kinetic Analyses of Data from a Human Serum Albumin Assay Using the liSPR System

January 1st, 2015|Comments Off on Kinetic Analyses of Data from a Human Serum Albumin Assay Using the liSPR System

Anja Henseleit , Carolin Pohl, Hans-Michael Kaltenbach, Karina Hettwer, Kirsten Simon, Steffen Uhlig, Natalie Haustein, Thomas Bley and Elke Boschke  We used the interaction between human serum albumin (HSA) and a high-affinity antibody to evaluate binding affinity measurements by the bench-top liSPR system (capitalis technology GmbH). HSA was immobilized directly onto a carboxylated sensor layer, and the mechanism of interaction between the antibody and HSA was investigated. The bivalence and heterogeneity of the antibody caused a complex binding mechanism. Three different interaction models (1:1 binding, heterogeneous analyte, bivalent analyte) were compared, [...]

1006, 2014

Development and assessment of a novel Arxula adeninivorans androgen screen (A-YAS) assay and its application in analysis of cattle urine

June 10th, 2014|Comments Off on Development and assessment of a novel Arxula adeninivorans androgen screen (A-YAS) assay and its application in analysis of cattle urine

Gerlach T, Knaust J, Kaiser C, Körner M, Hettwer , Uhlig S, Simon K, Baronian K, Kunze G The novel A-YAS assay for the detection of androgenic activity in liquid samples such as urine has been developed and assessed. The assay is based on transgenic Arxula adeninivorans yeast cells as the bio-component. The cells were engineered to co-express the human androgen receptor (hAR) gene and the inducible phytase reporter gene (phyK, derived from Klebsiella sp. ASR1), under the control of an Arxula derived glucoamylase (GAA) promoter, which had been modified by the insertion of hormone-responsive elements (HREs). The [...]

1508, 2013

Application of modified Arxula adeninivorans yeast cells in an online biosensor for the detection of estrogenic compounds in wastewater sample

August 15th, 2013|Comments Off on Application of modified Arxula adeninivorans yeast cells in an online biosensor for the detection of estrogenic compounds in wastewater sample

H.T.M. Pham, K. Kunath, L. Gehrmann, M. Giersberg, J. Tuerk, S. Uhlig, G. Hanke, K. Simon, K. Baronian, G. Kunze The ‘EstraMonitor’ was developed to provide an automated system for the detection of estrogenic compounds. It can be used for semi-online and continuous monitoring without additional instrumentation. Previously it was used to detect pure estrogenic molecules. In this investigation, the ‘EstraMonitor’ is used to detect the estrogenic activity in five non-pretreated wastewater samples. The ‘EstraMonitor’ employs genetically modified Arxula adeninivorans G1212/YRC102-hERα-phyK yeast cells as the detection component. Both immobilized [...]

101, 2012

EstraMonitor – A monitor for amperometric detection of estrogenic activity with Arxula adeninivorans yeast cells as the biocomponent

January 1st, 2012|Comments Off on EstraMonitor – A monitor for amperometric detection of estrogenic activity with Arxula adeninivorans yeast cells as the biocomponent

Pham H., Giersberg M., Uhlig S., Hanke G., Simon K., Kunath K., Baronian K., Kunze G. The EstraMonitor has been developed to provide a device suitable for monitoring estrogens in sewage treatment plants, i.e. provide rapid detection in semi-online operation. This monitor is based on recombinant Arxula adeninivorans yeast cells as the biocomponent. The cells were engineered to co-express the human estrogen receptor, (hER) gene and the inducible phytase (phyK, derived from Klebsiella sp. ASR1) reporter gene under the control of a promoter with estrogen response elements (EREs). [...]

Projects

Endocrine disruption in Antarctic waters

December 14th, 2016|Comments Off on Endocrine disruption in Antarctic waters

  Chemicals that exert endocrine effects in organisms, commonly referred to as endocrine-disrupting compounds (EDCs), have become a growing concern in recent years. Exposure to EDCs is currently regarded as one of the most serious anthropogenic threats to biodiversity and particularly to aquatic ecosystems through breeding disruption and fecundity reduction in a variety of marine species. EDCs are particularly harmful to reproductive and early development phases and the most commonly reported EDCs, namely estrogenic active compounds, have been associated with [...]