A-YDS Test-Kit for the detection of the Arylhydrocarbon (Ah) receptor mediated activity
In our uniquely developed A-YAS® (Arxula Yeast Androgen Screen) test method the best properties of all effect-based and not ELISA-related methods are combined. Hence, this test kit enables you to determine the estrogenic activity of aqueous samples already at a concentration of dioxin and dioxin-active substances in ng/L range.
The A-YDS intended use is the testing of substances. The cell-based screening method determines the activity of single substances and the cumulative activities of a substance mix which are mediated by Arylhydrocarbon (Ah) receptors. Sterile conditions are not required. Using standardized production conditions, we can ensure the consistent quality our test organism. The ready-to-use yeast offers the benefit of significantly reducing your workload. By using the recombinant test organism Arxula adeninivorans you can test your samples within ca. 26 hours for the presence of Ah-receptor affine ligands.
The A-YDS Test-Kit is suitable for the analysis of
- Surface water
and for substance testing
The A-YAS® test kit is easy-to-use, has a low-detection limit and comes with a free analysis software making it ideal for active ingredient research, in addition to various fields of application in biotechnology.
|Duration of assay||26 h|
|Number of samples (EEQ)||max. 40|
|Number of samples (LID)||max. 4|
|Calibration range *||0 – 10000|
|Limit of detection*||59|
* ng/L β-Naphthoflavon
The A-YDS test is based on a genetically modified strain of the A. adeninivorans yeast in which genome the gene for the human Arylhydrocarbon receptor (hAhR) as well as the gene for the human Arylhydrocarbon receptor nuclear trankslocator (hARNT) was integrated. If a substance with chemical affinity (ligand) is present, the reporter enzym (phytase) is produced throught the interaction of the ligand the receptor dimer hAH and hARNT. The amount of the reporter enzyme correlates to the total concentration of substances in the sample which interact with the receptors. With the addition of the chromogenic substrate p-nitrophenyl phosphate, the concentration of the reporter enzyme can now be measured. β-Naphthoflavone is used as a reference standard for calibration and quantification.
Ligand = AhR active substance / AhR = Arylhydrocarbon Receptor / TEFp = Translations-Elongationsfaktor Promotor / hAhR gen = gen for human Arylhydrocarbon Receptor / ARNT = Aryl-Hydrocarbon-Rezeptor Nuklear Translocator / GAAp = Glucoamylase Promotor / XRE = Xenobiotic Responsives Element / phyK gen = Phytase reporter gen / P = Phytase.