In our uniquely developed A-YAS® (Arxula Yeast Androgen Screen) test method the best properties of all effect-based and not ELISA-related methods are combined. Hence, this test kit enables you to determine the estrogenic activity of aqueous samples already at a concentration of androgens and androgenic substances in ng/L range.

The robust measurement method is insusceptible to microbial flora and therefore can be applied to complex samples without significant pretreatment (for example, samples from sewage treatment plants or those with high salt content).  Sterile conditions are not required. Using standardized production conditions, we can ensure the consistent quality our test organism. The ready-to-use yeast offers the benefit of significantly reducing your workload. The recombinant test organism Arxula adeninivorans can handle samples that have different salinity and are spiked with androgenic substances on a single plate within approximately 26 hours.

The A-YAS® Test-Kit is suitable for the analysis of

A-YAS® Test-Kit for the detection of androgenic substances

  • Ultrapure, drinking and mineral water
  • Surface water
  • Ground water and well water
  • Sewage
  • Seawater and brackish water
  • Aqueous extracts

  • and for substance testing

The A-YAS® test kit is easy-to-use, has a low-detection limit and comes with a free analysis software making it ideal for quality control in environmental and food analysis, in addition to various fields of application in biotechnology. Click here for more information on androgens and androgenic substances.

Duration of assayca. 26 h
Number of samples (EEQ)max. 40
Number of samples (LID)max. 4
Validationin-house
Calibration range *0 – 360
Limit of detection*31.3

* ng/L 5α-Dihydrotestosterone (DHT)

The A-YAS® test is based on a genetically modified strain of the A. adeninivorans yeast which has been modified to bind with the human estrogen receptor, hAR. Binding of androgenic substances to the receptor subsequently activates production of a secretory reporter enzyme. The amount of enzyme activity correlates to the total concentration of androgenic substances in the sample. With the addition of the chromogenic substrate p-nitrophenyl phosphate, enzyme activity can be measured photometrically. 5α-Dihydrotestosterone (DHT) is used as a reference standard for calibration and quantification.

TEFp = translations elongations factor promotor / hAR = human androgen receptor gene/ GAAp = glucoamylase promotor /        phyK = phytase reporter gene.

The detection is carried out photometrically after enzymatic reaction with a chromogenic substrate (p-nitrophenylphosphate).

The color-change reaction is catalyzed by the enzyme phytase:

Enzymatische Reaktion gelb EUSAAT Poster

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Every kit comes complete with BioVAL®

Datenanalyse-Software BioVAL

Get statistical analysis of your data in the form of a “Certificate of Analysis” with BioVAL®. All the important parameters from LOD to LOQ are conveniently summarized in a graph. QuoData software is not only reliable but also certified. Learn more here!

Your Contact

M. Sc. Martin JähneLaboratory Manager, Research & Development in Biotechnology
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