Products for a Cleaner Environment

The introduction of natural and anthropogenic trace substances with hormonal activity into the environment puts ecosystems at risk. Hormonal substances are especially threatening to aquatic organisms.

In 2008, Canada became the first country to label Bisphenol A (BPA), the starting material for the synthesis of polymer plastics, a hazardous substance and ban the use of it in baby bottles. This decision came as a result of numerous animal studies which showed the disruptive endocrine effects that BPA can have. In the following years, other countries such as France (2010) and Austria (2011) followed suit and in 2011, the German Federal Ministry of Food, Agriculture and Consumer Protection also banned the use of BPA in baby bottles.

A number of endocrine active substances such as 17β-estradiol (E2) and 17α-ethinyl estradiol (EE2), are currently on the European Union’s watch list of potentially dangerous substances. With continued experiments, these substances are gaining more attention from EU directives. In the formulation of environmental quality standards, there will most likely be restrictions placed on the use of other potential endocrine substances and the maximum concentrations that can be released into the environment. There is therefore a strong need for the development of strategies for the detection and elimination of endocrine active substances.

We believe that an important contribution can be made with biological test kits. Our kits are cost-effective, and can be used to quickly and efficiently test diverse samples for their hormonal content. Interested? See for yourself!

In-vitro test kits for the detection of hormonally-active substances in aqueous samples

With our in-vitro test kits, you can establish impact-based analysis for the detection of hormones and hormonally-active substances in your lab. The test kits that we create here at new_diagnostics GmbH, use the biological potential of the genetically modified yeast strain, Arxula adeninivorans, to ensure a consistent biosensing quality over several months of experiments. In our test kits we provide Arxula adeninivorans in a freeze-dried state as well as all the test media and buffers needed to run the assay. With the kit, you will also receive free access to our software, BioVAL® which you can use to plan and analyze your assay.

Arxula adeninivorans (Blastobotrys adeninivorans) is a unique temperature and salt tolerant yeast with a temperature-dependent reversible dimorphism. At temperatures above 42°C the yeast morphs into a filamentous structure. A. adeninivorans can tolerate ambient temperatures up to 55°C and, unlike conventional yeast, it can tolerate salinities of up to 200 PSU (Practical Salinity Units). This is equivalent to a 20% sodium chloride solution. Another feature of this yeast is its robust pH tolerance. A. adeninivorans can be cultivated in acidic medium with a pH as low as 2. These special physiological properties allow the use of this organism for testing samples with complex properties such as marine salt water or industrial process wastewater.

The measured effect of hormonally active substances in a sample depends on the specificity of the respective test kit. The scope of the assay is determined by the specific modification of the test organism, Arxula adeninivorans. A specific regulatory gene sequence and reporter were integrated into the genome of the yeast by one of our collaborators at IPK, Gatersleben. In the presence of a ligand, the designed ligand-receptor complex acts as a transcription factor to cause the formation of a reporter enzyme which is secreted into the extracellular medium. The amount of reporter enzyme produced correlates to the total concentration of the target substances. The enzyme is detected photometrically after the reaction with a chromogenic substrate. Quantification is then carried out by calibration with a reference standard.
If you want to use our A. adenivorans kits in your lab, please note that any work with genetically modified organisms must be carried out in a genetic S1 lab. If your laboratory does not have S1 status, we can support you in the accreditation process by providing you with the required information and documents.

To carry out the assay, the following laboratory equipment is required:

  • Centrifuge for Deepwell and Microtiter Plates
  • Shaking incubator system
  • Microliter centrifuge
  • One-channel pipette set
  • Multichannel pipette (10 – 300 ul)
  • Photometer for microtiter plates

Datasheet with Technical Requirements

With BioVAL®, our uncomplicated assay software, statistical evaluations can be carried out quickly and easily without prior statistical expertise. Thanks to the integrated planning module in BioVAL®, you can plan your plate design and pipetting scheme and customize the experiment. With the software’s robust statistics, semi-quantitative measurement methods and nonlinear calibrations can be analyzed. BioVAL® generates a sigmoidal curve to provide the necessary tools to evaluate your data from enzyme or receptor coupled measurement processes. These tools include powerful statistical methods to quantify:

  • Calibration
  • Measurement uncertainty
  • Detection and determination limits
  • Database
  • Evaluation charts

The analysis reports are available at any time in a number of different formats (Microsoft Word, Excel, PDF, and HTML).

By using the Xplor® 2 expression platform for the genetic modification of A. adeninivorans yeast the desired components (ex. human hormone receptors, reporter enzymes) can be integrated into the yeast genome quickly, effectively and stably. Thanks to the mitotic stability of the yeast, the danger of a change of test organism in response to testing conditions can be eliminated. Due to the abandonment of resistance markers and E. Coli sequences in the Arxual transformants, there is also no risk of horizontal gene transfer of antibiotic resistance. Finally, because A. adeninivorans is unable to form spores, there is no danger of uncontrolled yeast release by sporulation.

detections of estrogens

A-YES®

information about estrogens

detection of androgens

A-YAS®

information about androgens

detection of progestogens

A-YPS

information about progestagens