Speed-YESMD for the fast detection of estrogenic effects

The biological test system Speed-YESMD (Speed-Yeast Estrogen Screen) is a biological measurement system for the detection of the estrogenic activity in aqueous samples. The modified test is based on the conventional method YES (McDonnell et al. 1991)1,2 and our Speed-YESMD and is capable for a really fast assessment of the estrogenic potential of various water types.
The genetic modified ready-to-use yeast Saccharomyces cerevisiae (BJ3505) offers the benefit of significantly reduced workload and workflow. Due to the optimized test procedure, results for estrogenic potential are available after ca. six hours. The whole test can be performed under non-sterile conditions. By using standardized production conditions, we ensure the consistent quality of the test organism. Validation for the Speed-YESMD was accomplished with real water samples in a factorial in-house study.

1McDonnell DP et al.; High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae; Steroid Biochem. Mol. Biol.; Sep. 1991; 39(3); 291-297

2McDonnell DP et al.; In Situ Distinction between Steroid Receptor Binding and Transactivation at a Target Gene, Molecular and Cellular Biology; Sept. 1991; 11 (9); 4350-4355

The Speed-YESMD test is suitable for the analysis of:

  • Aqueous extracts

  • Ultrapure, drinking and mineral water

  • Surface water

  • Ground water and well water

  • Waste water

The Speed-YESMD test kit is ready-to-use with a low detection limit, making it ideal for quality control in environmental and food analysis and for various fields of application in biotechnology. Every kit comes with our software BioVAL® – the software tool for evaluation of your measurement data.

Click here for more information on estrogens and estrogenic substances.

Duration of assayca. 6 h
Number of Samples (EEQ)max. 80 (Speed-YESMD plus)
Validationin-house
Calibration range0 – 400 ng/L 17β-Estradiol (E2)
Limit of detection6.8 ng/L 17β-Estradiol (E2)

The detection is carried out photometrically after enzymatic reaction with the chromogenic substrate chlorophenol red-β-D-galactopyranoside.

Catalysed reaction by the enzyme β-galactosidase::

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Every kit comes complete with BioVAL®

Get statistical analysis of your data in the form of a “Certificate of Analysis” with BioVAL®. QuoData software is not only reliable but also certified. Learn more here!

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