S-YESMD for the detection of estrogenic effects
The biological test system S-YESMD (Saccharomyces-Yeast Estrogen Screen) is a biological measurement system for the detection of the estrogenic activity in aqueous samples. The modified test is based on the conventional method YES (McDonnell et al. 1991)1,2 and is capable in particular for screening purposes of surface water and extracts with organic solvent content up to 15 %3.
The ready-to-use yeast Saccharomyces cerevisiae (BJ3505) offers the benefit of significantly reduced workload and workflow. The whole test can be performed under non-sterile conditions. By using standardized production conditions, we ensure the consistent quality of the test organism. Validation for the S-YESMD was accomplished with real water samples in a factorial in-house study.
1McDonnell DP et al.; High level expression of biologically active estrogen receptor in Saccharomyces cerevisiae; Steroid Biochem. Mol. Biol.; Sep. 1991; 39(3); 291-297
2McDonnell DP et al.; In Situ Distinction between Steroid Receptor Binding and Transactivation at a Target Gene, Molecular and Cellular Biology; Sept. 1991; 11 (9); 4350-4355
3dependent on the used organic solvent.
The S-YESMD test is suitable for the analysis of:
Aqueous extracts with solvent concentration up to 15 %
Ultrapure, drinking and mineral water
Surface water
Ground water and well water
Waste water
The S-YESMD test kit is ready-to-use with a low detection limit, making it ideal for quality control in environmental and food analysis and for various fields of application in biotechnology. Every kit comes with our software BioVAL® – the software tool for evaluation of your measurement data.
Click here for more information on estrogens and estrogenic substances.
| Duration of assay | ca. 20 h |
| Number of Samples (EEQ) | max. 80 (S-YESMD plus) |
| Validation | in-house |
| Calibration range | 0 – 400 ng/L 17β-Estradiol (E2) |
| Limit of detection | 3.8 ng/L 17β-Estradiol (E2) |
The detection is carried out photometrically after enzymatic reaction with the chromogenic substrate chlorophenol red-β-D-galactopyranoside.
Catalysed reaction by the enzyme β-galactosidase::
Every kit comes complete with BioVAL®
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