µA-YES for the detection of estrogenic effects

The biological test system μA-YES (microArxula-Yeast Estrogen Screen) based on our A-YES® and is designed for 384 well plates. The test is an effect-directed, yeast cell-based assay for a highly sensitive detection of estrogenic activity in all types of aqueous samples including saline water, wastewater and environmental water. The test is particular applicable for samples with limited sample volume such as extracts due to the low sample volume of 40 µl. The μA-YES measures the cumulative estrogenic activity of a sample in a fast, easy, economic and reliable manner. With the μA-YES you can determine the estrogenic effect of a sample (EEQ) as well as the the dilution level at which an estrogenic effect does no longer occur (LID – lowest ineffective dilution). The μA-YES is equivalent to the ISO method A-YES® (ISO 19040-2).

The ready-to-use yeast Arxula adeninivorans offers the benefit of significantly reduced workload and workflow. By using standardized production conditions, we ensure the consistent quality of our test organism.

The µA-YES test kit is suitable for the analysis of:

  • Aqueous extracts

  • Ultrapure, drinking and mineral water

  • Surface water

  • Ground water and well water

  • Wastewater

  • Seawater and brackish water

Because of the low detection limit and the easy-to-use the µA-YES test kit is ideal for quality control in environmental and food analysis and also for various fields of application in biotechnology. Every kit comes with our software BioVAL® – the software tool for evaluation of your measurement data.

Click here for more information on estrogens and estrogenic substances.

Duration of assayca. 26 h
Number of Samples(EEQ)max. 80
Number of Samples(LID)max. 8
Validationinterlaboratory trial
Calibration range*0 – 80
Limit of Detection*2.3

* ng/L  17β-Estradiol (E2)

The A-YES® test is based on a genetically modified strain of the A. adeninivorans yeast which has been modified to bind with the human estrogen receptor, hERα. Binding of androgenic substances to the receptor subsequently activates production of a secretory reporter enzyme. The amount of enzyme activity correlates to the total concentration of estrogenic substances in the sample. With the addition of the chromogenic substrate p-nitrophenyl phosphate, enzyme activity can be measured photometrically. 17 β-estradiol (E2) is used as a reference standard for calibration and quantification.

TEFp = translation-elongation factor promotor / hERα = human estrogen receptor alpha gene / GAAp = glucoamylase promotor / phyK = phytase reporter gene

The detection is carried out photometrically after enzymatic reaction with a chromogenic substrate (p-nitrophenylphosphate).

The color-change reaction is catalyzed by the enzyme phytase:

Enzymatische Reaktion gelb EUSAAT Poster

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Every kit comes complete with BioVAL®

Datenanalyse-Software BioVAL

Get statistical analysis of your data in the form of a “Certificate of Analysis” with BioVAL®. All the important parameters from LOD to LOQ are conveniently summarized in a graph. QuoData software is not only reliable but also certified. Learn more here!

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