µA-YES for the detection of estrogenic effects
The biological test system μA-YES (microArxula-Yeast Estrogen Screen) based on our A-YES® and is designed for 384 well plates. The test is an effect-directed, yeast cell-based assay for a highly sensitive detection of estrogenic activity in all types of aqueous samples including saline water, wastewater and environmental water. The test is particular applicable for samples with limited sample volume such as extracts due to the low sample volume of 40 µl. The μA-YES measures the cumulative estrogenic activity of a sample in a fast, easy, economic and reliable manner. With the μA-YES you can determine the estrogenic effect of a sample (EEQ) as well as the the dilution level at which an estrogenic effect does no longer occur (LID – lowest ineffective dilution). The μA-YES is equivalent to the ISO method A-YES® (ISO 19040-2).
The ready-to-use yeast Arxula adeninivorans offers the benefit of significantly reduced workload and workflow. By using standardized production conditions, we ensure the consistent quality of our test organism.
The µA-YES test kit is suitable for the analysis of:
Ultrapure, drinking and mineral water
Ground water and well water
Seawater and brackish water
Because of the low detection limit and the easy-to-use the µA-YES test kit is ideal for quality control in environmental and food analysis and also for various fields of application in biotechnology. Every kit comes with our software BioVAL® – the software tool for evaluation of your measurement data.
Click here for more information on estrogens and estrogenic substances.
|Duration of assay||ca. 26 h|
|Number of Samples(EEQ)||max. 80|
|Number of Samples(LID)||max. 8|
|Calibration range*||0 – 80|
|Limit of Detection*||2.3|
* ng/L 17β-Estradiol (E2)
The µA-YES is based on the genetically modified yeast A. adeninivorans. In the yeast genome the gene for the human estrogen receptor (hERα) and a reporter gene cassette have been integrated. Estrogens and estrogenically active substances bind as ligands to the receptors and the resulting complexes act as transcription factors for the transcription of the reporter gene. The amount of reporter produced enzyme correlates with the total concentration of estrogen-active substances in the sample. Detection is performed photometrically after enzymatic reaction with the chromogenic substrate p-nitrophenyl phosphate. The reference standard for calibration and quantification is 17β-Estradiol (E2).