A-YAS® Test kit for the detection of androgenic effects
In our uniquely developed A-YAS® (Arxula Yeast Androgen Screen) test kit, the best properties of all effect-based methods are combined. This non-ELISA based method enables you to determine the concentration of androgens and androgenic substances in aqueous samples to the precision of single-digit ng/L range.
This robust measurement method is insusceptible to microbial flora and therefore can be applied to complex samples without significant pretreatment (for example, samples from sewage treatment plants or those with high salt content). Moreover, sterile conditions are not required. The ready-to-use yeast offers the benefit of significantly reducing workload and worflow. The recombinant test organism Arxula adeninivorans can handle samples that have different salinity and are spiked with androgenic substances on a single plate within approximately 26 hours. By using standardized production conditions, we ensure the consistent quality of our test organism.
The A-YAS® Test kit is suitable for the analysis of
- Ultrapure, drinking and mineral water
- Surface water
- Ground water and well water
and for substance testing
The A-YAS® test kit has a low detection limit and is extremely easy-to-use and comes with free analysis software BioVAL® making it ideal for quality control in environmental and food analysis, in addition to various fields of application in biotechnology. Click here for more information on androgens and androgenic substances.
|Duration of assay||ca. 26 h|
|Number of samples (DHTEQ)||max. 40|
|Calibration range *||0 – 360|
|Limit of detection*||31.3|
* ng/L 5α-Dihydrotestosterone (DHT)
The A-YAS® is based on the genetically modified yeast A. adeninivorans. In the yeast genome the gene for the human androgen receptor (hAR) and a reporter gene cassette have been integrated. Androgens and androgenically active substances bind as ligands to the receptors and the resulting complexes act as transcription factors for the transcription of the reporter gene. The amount of reporter produced enzyme correlates with the total concentration of androgen-active substances in the sample. Detection is performed photometrically after enzymatic reaction with the chromogenic substrate p-nitrophenyl phosphate. The reference standard for calibration and quantification is 5α-Dihydrotestosterone (DHT).
TEF = translations elongations factor promotor / hAR = human androgen receptor gene/ GAA = glucoamylase promotor / phyK = phytase reporter gene.