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A-YDS Test kit for the detection of the Arylhydrocarbon (Ah) receptor mediated effects

In our uniquely developed A-YDS test kit, the best properties of all effect-based methods are combined. This Non-ELISA based method enables you to determine the concentration of dioxin and dioxin-active substances in ng/L range.

This cell-based screening method determines the activity of single substances and the cumulative activities of a substance mix which are mediated by Arylhydrocarbon (Ah) receptors. It is intended to be used for testing of substances like meat and fish. Sterile conditions are not required for this test. The ready-to-use yeast offers the benefit of significantly reducing your workload and workflow. By using the recombinant test organism Arxula adeninivorans you can test your samples within approximately 26 hours for the presence of Ah-receptor affine ligands. Using standardized production conditions, we ensure the consistent quality our test organism.

The A-YDS Test-Kit is suitable for the analysis of

  • Aqueous extracts

  • Surface water
  • and for substance testing

The A-YDS test kit is easy-to-use, has a low detection limit and comes with a free analysis software BioVAL® making it ideal for active ingredient research, in addition to various fields of application in biotechnology.

Duration of assay26 h
Number of samples (NEQ)max. 40
Calibration range *0 – 10000
Limit of detection*59

* ng/L β-Naphthoflavon

The A-YDS test is based on a genetically modified strain of the A. adeninivorans yeast in which genome the gene for the human Arylhydrocarbon receptor (hAhR) as well as the gene for the human Arylhydrocarbon receptor nuclear trankslocator (hARNT) was integrated. If a substance with chemical affinity (ligand) is present, the reporter enzym (phytase) is produced throught the interaction of the ligand the receptor dimer hAH and hARNT.  The amount of the reporter enzyme correlates to the total concentration of substances in the sample which interact with the receptors. With the addition of the chromogenic substrate p-nitrophenyl phosphate, the concentration of the reporter enzyme can now be measured. β-Naphthoflavone is used as a reference standard for calibration and quantification.

Ligand = AhR active substance / AhR = Arylhydrocarbon Receptor / TEFp = Translations-Elongationsfaktor Promotor / hAhR gen = gen for human Arylhydrocarbon Receptor / ARNT = Aryl-Hydrocarbon-Rezeptor Nuklear Translocator / GAAp = Glucoamylase Promotor /  XRE = Xenobiotic Responsives Element /  phyK gen = Phytase reporter gen /  P = Phytase.

The detection is carried out photometrically after enzymatic reaction with a chromogenic substrate (p-nitrophenylphosphate).

The color-change reaction is catalyzed by the enzyme phytase:

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Every kit comes complete with BioVAL®

Datenanalyse-Software BioVAL

Get statistical analysis of your data in the form of a “Certificate of Analysis” with BioVAL®. All the important parameters from LOD to LOQ are conveniently summarized in a graph. QuoData software is not only reliable but also certified. Learn more here!

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