A-YBS for the detection of bisphenols
The biological test system A-YBS (Arxula-Yeast Bisphenol Screen) based on our well established A-YES®. The A-YBS is an effect-directed, yeast cell-based assay for a highly sensitive and specific detection of bisphenols in all types of aqueous samples. By directed modification1 of the DNA sequence of the human estrogen receptor α gene the specificity and sensitivity for bisphenols, especially bisphenol A and bisphenol Z, is increased. The specificity and sensitivity for natural estrogens is reduced significantly.
With the robust assay you are able to measure bisphenols in aqueous leachates and extracts e.g. from plastic ware and coatings or in environmental samples such as wastewater. The ready-to-use yeast Arxula adeninivorans offers the benefit of significantly reduced workload and workflow. By using standardized production conditions, we ensure the consistent quality of our test organism. The validity of the assay was proven by a factorial in-house validation study with real samples.
The A-YBS test kit is suitable for the analysis of
- Ultrapure, drinking and mineral water
- Surface water
- Ground water and well water
Because of the low detection limit, the easy-to-use the A-YBS test kit is ideal for quality control in environmental and food analysis and also for various fields of application in biotechnology. Every kit comes with our software BioVAL® – the software tool for evaluation of your measurement data.
1Johanna Rajasärkkä, Marko Virta; Characterization of a Bisphenol A Specific Yeast Bioreporter Utilizing the Bisphenol A-Targeted Receptor; Anal. Chem., 2013, 85 (21), pp 10067–10074
|Duration of assay||ca. 26 h|
|Number of Samples (BPAEQ)||max. 40|
|Calibration range*||0 – 100|
|Limit of Detection*||6.4|
*µg/L Bisphenol A (BPA)
TEF = translation-elongation factor promotor / hERα = human estrogen receptor alpha gene / GAA = glucoamylase promotor / phyK = phytase reporter gene.