A-YBS for the detection of bisphenols

The biological test system A-YBS (Arxula-Yeast Bisphenol Screen) based on our well established A-YES®. The A-YBS is an effect-directed, yeast cell-based assay for a highly sensitive and specific detection of bisphenols in all types of aqueous samples. By directed modification1 of the DNA sequence of the human estrogen receptor α gene the specificity and sensitivity for bisphenols, especially bisphenol A and bisphenol Z, is increased. The specificity and sensitivity for natural estrogens is reduced significantly.

With the robust assay you are able to measure bisphenols in aqueous leachates and extracts e.g. from plastic ware and coatings or in environmental samples such as wastewater. The ready-to-use yeast Arxula adeninivorans offers the benefit of significantly reduced workload and workflow. By using standardized production conditions, we ensure the consistent quality of our test organism. The validity of the assay was proven by a factorial in-house validation study with real samples.

The A-YBS test kit is suitable for the analysis of

  • Aqueous extracts

  • Ultrapure, drinking and mineral water
  • Surface water
  • Ground water and well water
  • Wastewater

Because of the low detection limit, the easy-to-use the A-YBS test kit is ideal for quality control in environmental and food analysis and also for various fields of application in biotechnology. Every kit comes with our software BioVAL® – the software tool for evaluation of your measurement data.

1Johanna Rajasärkkä, Marko Virta; Characterization of a Bisphenol A Specific Yeast Bioreporter Utilizing the Bisphenol A-Targeted Receptor; Anal. Chem., 2013, 85 (21), pp 10067–10074

Duration of  assayca. 26 h
Number of Samples (BPAEQ)max. 40
Validationin-house
Calibration range*0 – 100
Limit of Detection*6.4

*µg/L Bisphenol A (BPA)

The A-YBS is based on the genetically modified yeast A. adeninivorans. In the yeast genome, the modified gene for the human estrogen receptor hERα and a reporter gene cassette have been integrated. Bisphenols bind as ligands to the receptors and the resulting complexes act as transcription factors for the transcription of the reporter gene. The amount of produced reporter enzyme correlates with the total concentration of bisphenols in the sample. The detection is performed photometrically after enzymatic reaction with the chromogenic substrate p-nitrophenylphosphate. Bisphenol A is used as reference standard for calibration and quantification.

TEF = translation-elongation factor promotor / hERα = human estrogen receptor alpha gene / GAA = glucoamylase promotor / phyK = phytase reporter gene.

Request an A-YBS offer

Every kit comes complete with BioVAL®

Get statistical analysis of your data in the form of a “Certificate of Analysis” with BioVAL®. QuoData software is not only reliable but also certified. Learn more here!

Contact us for further information