In-vitro test kits for the detection of hormonally-active substances in aqueous samples

With our in vitro tests, it is possible to establish effect-directed analysis for the detection of hormones and hormonally active substances in your laboratory. The tests of new_diagnostics use the biological potential of the genetically modified yeast Arxula adeninivorans and Saccharomyces cerevisiae. To ensure consistent biosensor quality for several months we provide the yeast strains in our test kits in a freeze-dried condition, as well as all test media and buffers needed to run the assay. In combination with our tests you receive free access to our software BioVAL® for evaluation of your measurement data.

Arxula adeninivorans (Blastobotrys adeninivorans) is a unique temperature and salt tolerant yeast with a temperature-dependent reversible dimorphism. At temperatures above 42°C the yeast morphs into a filamentous structure. A. adeninivorans can tolerate ambient temperatures up to 55°C and, unlike conventional yeast, it can tolerate salinities of up to 200 PSU (Practical Salinity Units). This is equivalent to a 20% sodium chloride solution. Another feature of this yeast is its robust pH tolerance. A. adeninivorans can be cultivated in acidic medium with a pH as low as 2. These special physiological properties allow the use of this organism for testing samples with complex properties such as marine salt water or industrial process wastewater. A. adeninivorans is unable to form spores. There is no risk of uncontrolled release of yeast by sporulation.

Saccharomyces cerevisiae ZellenSaccharomyces cerevisiae is facultative anaerobic yeast with round, oval shaped cells. The cells are stretched and form pseudomycel under defined environmental conditions. S. cerevisiae is able to produce ethanol by fermentation of different kinds of sugars (e.g. glucose, sucrose, maltose). The fermentation process runs until a concentration of ethanol of 18 % (v/v) is reached in the medium. Thus, the yeast allows the analysis of samples with high organic solvent content. The reproduction of S. cerevisiae can be carried out whether yeast are in a vegetative or sexual mode.

The measured effect of hormonally active substances in a sample depends on the specificity of the respective test kit. Our tests are based on the yeast Arxula adeninivorans and Saccharomyces cerevisiae. By application of a specific receptor gene cassette, regulation sequences and a reporter gene cassette has been integrated into both the yeast genome (A. adeninivorans), or plasmids (S. cerevisiae) by one of our collaborators at IPK, Gatersleben. In the presence of a ligand, the designed ligand-receptor complex acts as a transcription factor to cause the formation of a reporter enzyme which is secreted into the extracellular medium. The amount of reporter enzyme produced correlates to the total concentration of the target substances. The enzyme is detected photometrically after the reaction with a chromogenic substrate. Quantification is then carried out by calibration with a reference standard.
We give sufficient information and support for the establishment of our test in your laboratory. Please regard the safety instructions of your country for genetically modified microorganisms if you wish to use our yeast based tests.

The following special equipment is required for performing our tests:

  • Microtiter plate centrifuge (only for selected adeninivorans based tests)
  • Temperature-controlled shaker for Deepwell plates
  • Multichannel pipette (nominal volume 10 – 300 μl)
  • Photometer for Microtiter plates

Datasheet with Technical Requirements

detections of estrogens

detection of androgens

detection of progestogens

detection of Ah-receptor mediated effects