Erik Böer, Gerhard Steinborn, Kristina Florschütz, Martina Körner, Gerd Gellissen, Gotthard Kunze
The dimorphic ascomycetous yeast Arxula adeninivorans exhibits some unusual properties. Being a thermo- and halotolerant species it is able to assimilate and ferment many compounds as sole carbon and/or nitrogen source. It utilises n-alkanes and is capable of degrading starch. Due to these unusual biochemical properties A. adeninivorans can be exploited as a gene donor for the production of enzymes with attractive biotechnological characteristics. Examples of A. adeninivorans-derived genes that are overexpressed include the ALIP1 gene encoding a secretory lipase, the AINV encoding invertase, the AXDH encoding xylitol dehydrogenase and the APHYencoding a secretory phosphatase with phytase activity.
The thermo- and halotolerance as well as differential morphology-dependent glycosylation and the secretion characteristics render A. adeninivorans attractive as host for heterologous gene expression. A transformation system has been established based on homologous integration of linearised DNA fragments. Successful expression examples like that of the E. coli-derived lacZgene, GFP and human HSA and IL6 genes add to the attraction of A. adeninivorans as host for hetero logous gene expression.