The determination of pharmaceuticals in wastewater using a recombinant Arxula adeninivorans whole cell biosensor

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The determination of pharmaceuticals in wastewater using a recombinant Arxula adeninivorans whole cell biosensor

Ha Thi Minh Pham, Martin Giersberg, Linda Gehrmann, Karina Hettwer, Jochen Tuerk, Steffen Uhlig, Gerold Hanke, Peter Weisswange, Kirsten Simon, Keith Baronian, Gotthard Kunze

An Arxula adeninivorans based microbial biosensor has been developed for the determination of pharmaceuticals and chemicals such as omeprazole, lansoprazole, β-naphtoflavone and methylcholanthrene within 5 h using biochemical detection and 4 h and 10 min using amperometric detection. The biosensor consists of genetically modifiedA. adeninivorans G1212/YRC102-hAhR-hARNT-phyK (hAhR – human arylhydrocarbon receptor; hARNT – human arylhydrocarbon receptor nuclear translocator; inducible phyK– derived from Klebsiella sp. ASR1) as the biological component and coupled with either a biochemical or an amperometric detection method. The combination between hAhreceptor gene, hARNT and the A. adeninivorans-derived glucoseamylase promoter of the reporter gene (GAA) containing specific cyp1A1-derived core sequence created a construct which enabled specific induction by pharmaceuticals. This offers a new cell-based biosensor for the pharmaceutical determination. The half maximum effective concentration (EC50) and the limit of detection (LoD) were found to be 236.13, 95.01 and 174.72, 83.65 μg/l for omeprazole and lansoprazole, respectively. These two pharmaceuticals are among the most widely used internationally. Additionally A. adeninivorans G1212/YRC102-hAhR-hARNT-phyK cells allow the measurement in raw wastewater, i.e. not concentrated, unpurified and untreated which will allow on-site operation in sewage treatment plants.

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